Reviews and Protocols in DT40 Research

FOREWORD Chapter 1. DT40 GENE DISRUPTIONS: A HOW-TO FOR THE DESIGN AND THE CONSTRUCTION OF TARGETING VECTORS Hiroshi Arakawa and Jean-Marie Buerstedde CHAPTER 2. IMMUNOGLOBULIN GENE CONVERSION or HYPERMUTATION: That's the question Jean-Marie Buerstedde and Hiroshi Arakawa CHAPTER 3. Genome resources for the dt40 community Randolph B Caldwell and Andrzej M. Kierzek CHAPTER 4. Chromosome engineering in DT40 cells and mammalian centromere function William RA Brown1, Margaret CM Smith2, Felix Dafhnis-Calas, Sunir Malla and Zhengyao Xu. CHAPTER 5. FUNCTION OF RECQ FAMILY HELICASE IN GENOME STABILITY Masayuki Seki, Shusuke Tada and Takemi Enomoto chapter 6. Genetic analysis of apoptotic execution Sandrine Ruchaud, Kumiko Samejima, Damien Hudson, Scott H. Kaufmann and William C. Earnshaw CHAPTER 7. The DT40 system as a tool for analyzing kinetochore assembly Masahiro Okada, Tesuya Hori and Tatsuo Fukagawa CHAPTER 8. Analysing the DNA damage and Replication Checkpoints in DT40 cells Michael D Rainey, George Zachos, and David AF Gillespie CHAPTER 9. USING DT40 TO STUDY CLATHRIN FUNCTION Frank R. Wettey and Antony P. Jackson CHAPTER 10. GENETIC ANALYSIS OF B CELL SIGNALING Hisaaki Shinohara and Tomohiro Kurosaki CHAPTER 11. DT40 MUTANTS: A MODEL TO STUDY transcriptional regulation of B CELL DEVELOPMENT AND FUNCTION Jukka Alinikula, Olli Lassila and Kalle-Pekka Nera CHAPTER 12. TRANSCRIPTION AND RNA PROCESSING FACTORS PLAY COMPLEX TOLES IN DT40 CELLS Stephanie Bush and James L. Manley CHAPTER 13. PARTICIPATION OF HISTONES, HISTONE MODIFYING ENZYMES AND HISTONE CHAPERONES IN VERTEBRATE CELL FUNCTIONS Hidehiko Kikuchi, Hirak Kumar Barman, Masami Nakayama, Yasunari Takami and Tatsuo Nakayama CHAPTER 14. ANALYSIS OF GENE EXPRESSION, COPY NUMBER AND PALINDROME FORMATION WITH A DT40 ENRICHED cDNA Microarray Paul E. Neima1, Joan Burnside, Katrina Elsaesser, Harry Hwang, Bruce E. Clurman, Robert Kimmel and Jeff Delrow
cHAPTER 15. Analysis of DNA replication damage bypass and its role in immunoglobulin repertoire development Julian E. Sale, Anna-Laura Ross and Laura J. Simpson CHAPTER 16. THE FANCONI ANEMIA PATHWAY PROMOTES HOMOLOGOUS RECOMBINATION REPAIR IN DT40 CELL LINE Julian E. Sale, Anna-Laura Ross and Laura J. Simpson CHAPTER 17. CALCIUM SIGNALING, ION CHANNELS AND MORE Anne-Laure Perraud, Carsten Schmitz, Andrew M. Scharenberg CHAPTER 18. Phenotypic analysis of Cellular responses to DNA damage Helfrid Hochegger and Shunichu Takeda CHAPTER 19. ATM, A PARADIGM FOR A STRESS-RESPONSIVE SIGNAL TRANSDUCER IN HIGHER VERTEBRATE CELLS Ken-ichi Yamamoto, Masahiko Kobayashi and Hiroko Shimizu METHOD 1. Stable transfection of DT40 Julian E. Sale METHOD 2. BASIC CELL CULTURE CONDITIONS Huseyin Saribasak and Hiroshi Arakawa METHOD 3. EXCISION OF FLOXED-DNA SEQUENCES BY TRANSIENT INDUCTION OF MER-CRE-MER Hiroshi Arakawa METHOD 4. IMMUNOGLOBULIN GENE CONVERSION AND HYPERMUTATION ASSAY BY FACS Hiroshi Arakawa METHOD 5. TARGET SCREENING BY PCR Hiroshi Arakawa METHOD 6. MITOTIC INDEX DETERMINATION BY FLOW CYTOMETRY David AF Gillespie and Mark Walker METHOD 7. Centrifugal elutriation as a means of cell cycle phase separation and synchronisation David AF Gillespie and Catarina Henriques METHOD 8. preparation of genomic dna for microarray-based comparative genome hybridization Robert Kimmel, Amalia Icreverzi, Paul Neiman METHOD 9. Analysis of cellular Mg2+ in DT40 cells Anne-Laure Perraud, Carsten Schmitz, Andrew M. Scharenberg METHOD 10. Transient transf