Preface<br>Lorenzo Galluzzi, Fernando Aranda, Aitziber Buque and José Manuel Bravo-San Pedro<br>1. Characterization of Mutants of the Fish Pathogen Flavobacterium psychrophilum.<br>Mario García Domínguez<br>2. Cannulation of mesenteric lymphatic vessels in a murine model<br>Zohaib N. Khan<br>3. Mouse intestine as a useful model for CFTR electrophysiology function analysis<br>Maria Chiara Maiuri<br>4. An immunocompetent mouse model of metastatic triple-negative breast cancer<br>Mohamed Jemaa<br>5. Generation of Humanized Bone for Disease Modeling Using Porcine Adipose Tissue-Derived Extracellular Matrix Scaffolds and Human Dental Pulp Stem Cells<br>Gaskon Ibarretxe, Iratxe Madarieta, Jon Luzuriaga, Nerea García-Urkia, Jone Salvador-Moya, Beatriz Pardo-Rodríguez, Patricia García Gallastegui, Verónica Uribe-Etxebarria, Francisco-Javier Fernández-San-Argimiro, Igor Irastorza, Lucía Jiménez-Rojo, Beatriz Olalde, Fernando Unda and José Ramón Pineda<br>6. The acute 6-OHDA mouse model<br>Jose A. Morales-Garcia<br>7. Methodology and evaluation of the induction of experimental autoimmune encephalomyelitis, a murine preclinical model of multiple sclerosis<br>Maria Luisa Nieto<br>8. Precise Measurement of Motor Neuron Dysfunction in Drosophila ALS Model via Climbing Assay and Leg Imaging<br>Yanan Wei, Hongyu Miao, Hadi Najafi and Woo Jae Kim<br>9. Evaluation of in vivo target cell elimination by antigen-specific cytotoxic T lymphocytes<br>Gustavo P. Amarante-Mendes, Abolaji S. Olagunju, Isau H. Noronha and José Ronnie C. Vasconcelos<br>10. A protocol for measuring phenotypical facial disease markers in a mouse model of iatrogenic Cushing’s syndrome.<br>Oliver Kepp, Guido Kroemer, Hui Pan, Ai-Ling Tian and Isabelle Martins<br>11. Swiss Webster mice as a model of alcohol overconsumption<br>Mohamed Jemaa<br>12. Orthotopic model of tongue cancer for the study of head and neck squamous cell carcinoma in mice<br>Maria Chiara Maiuri, Jonathan Pol, Guido Kroemer, Sarah Adriana Scuderi, Fatima Domenica Elisa De Palma, Deborah Mannino, Maria Perez-Lanzon, Lionel Deroche, Marie Valet and Pierre Cordier<br>13. Experimental model of Unilateral Ureteral Obstruction reproduces key events of chronic kidney disease: from mice to humans.cide<br>Lara Valiño, Alberto Lázaro Fernández, Elena Vázquez Ogando and María ángeles González Nicolas<br>14. Animal Models of Obesity<br>Eugenia Morselli<br>15. Methods for experimentally increasing circulating acyl coenzyme A binding protein (ACBP) levels in mice under chronic restraint stress<br>ISABELLE MARTINS<br>16. Assessing mitochondrial number and morphology in a C. elegans model of human tauopathy<br>Konstantinos Palikaras